豚鼠空肠肠神经丛中垂体腺苷酸环化酶激活多肽（PACAP）和血管活性肠肽（VIP）定位的免疫组织化学比较

Immunohistochemical comparison of localization of pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) in the enteric nerve plexus of the guinea pig jejunum.

作者信息

Suzuki M, Nokihara K, Ando E, Naruse S

机构信息

Department of Food and Nutrition, Yamanashi-Gakuin Junior College, Japan.

出版信息

Biomed Pept Proteins Nucleic Acids. 1996;2(1):19-22.

PMID:9346832

Abstract

The localization and co-localization of pituitary adenylate cyclase activating polypeptides (PACAPs) and vasoactive intestinal polypeptide (VIP) in the enteric nerve plexus of the guinea pig jejunum were immunohistochemically compared by the peroxidase anti-peroxidase (PAP) method using region-specific antisera against PACAP38, PACAP27 and VIP, respectively. Immunoreactive nerve elements were demonstrated in the ganglia of both myenteric and submucous plexi in the guinea pig jejunum. Numerous immunoreactive nerve cell bodies were localized in the myenteric ganglia, while nerve cell bodies in the submucous ganglia were only slightly immunopositive. Immunoreactive nerve terminal varicosities occurred in both myenteric and submucous ganglia. Additionally, PACAPs-immunoreactive nerve cell bodies were also immunopositive with VIP. Thus, both PACAP38 and PACAP27, as well as VIP, are co-localized in the cell bodies of guinea pig jejunum.

摘要

分别使用针对垂体腺苷酸环化酶激活多肽（PACAPs）38、PACAP27和血管活性肠肽（VIP）的区域特异性抗血清，通过过氧化物酶抗过氧化物酶（PAP）方法，免疫组织化学比较豚鼠空肠肠神经丛中PACAPs和VIP的定位及共定位情况。在豚鼠空肠的肌间神经丛和黏膜下神经丛的神经节中均显示出免疫反应性神经成分。大量免疫反应性神经细胞体定位于肌间神经节，而黏膜下神经节中的神经细胞体仅有轻微免疫阳性。免疫反应性神经终末膨体出现在肌间和黏膜下神经节中。此外，PACAPs免疫反应性神经细胞体对VIP也呈免疫阳性。因此，PACAP38和PACAP27以及VIP均共定位于豚鼠空肠的细胞体中。