Sarroca A, Lin S, Bonanno J A
Morton D. Sarver Center for Cornea and Contact Lens Research, University of California, School of Optometry, Berkeley 94720-2020, USA.
CLAO J. 1997 Oct;23(4):282-5.
Hypoxic corneal swelling in human subjects is reduced by CO2 induced corneal acidosis. We investigated whether this effect could be explained by a reduction in lactate production under acidic conditions.
Lactate production from isolated bovine corneas was assayed under normoxic and hypoxic conditions at normal pH (7.5) and low pH (7.05). Low pH was achieved using a CO2/HCO3- Ringer or a HEPES buffered Ringer.
Normoxic corneas produced significantly less (approximately 25%) lactate under all acidic conditions. In contrast, hypoxic corneas produced the same or slightly less lactate under acidic conditions (weighted average of all conditions = 6% less lactate).
From these results, we concluded that pH can have a substantial effect on lactate production, but its influence on glycolytic activity during hypoxia is suppressed and may be negligible.
二氧化碳诱导的角膜酸中毒可减轻人体受试者的缺氧性角膜肿胀。我们研究了这种效应是否可以通过酸性条件下乳酸生成的减少来解释。
在正常pH值（7.5）和低pH值（7.05）的常氧和缺氧条件下，测定离体牛角膜的乳酸生成。使用二氧化碳/碳酸氢根林格液或羟乙基哌嗪乙磺酸缓冲林格液实现低pH值。
在所有酸性条件下，常氧角膜产生的乳酸显著减少（约25%）。相比之下，缺氧角膜在酸性条件下产生的乳酸相同或略有减少（所有条件的加权平均值 = 乳酸减少6%）。
根据这些结果，我们得出结论，pH值可对乳酸生成产生实质性影响，但其在缺氧期间对糖酵解活性的影响受到抑制，可能微不足道。
