Mainguené C, Choquenet C, Deplano C, Gavelli A, Clément N, Vanzo E, Hofman P
Department of Pathology, Hôpital Princesse Grace, Monaco.
Anal Quant Cytol Histol. 1997 Oct;19(5):437-42.
To compare DNA content measured by image cytometry from touch imprints and formalinfixed, paraffin-embedded samples in bladder carcinomas.
Thirty-one biopsies of urothelial carcinomas were selected for a prospective study. Imprints of fresh specimens were performed. Cell suspensions were obtained from dewaxed samples by the procedure of Hedley. Sections 7 microns thick were used for carcinoma in situ and small biopsies. The DNA ploidy index was measured on Feulgen-stained slides using an image cytometer.
From imprint analysis, seven grade 1 carcinomas (n = 9) were found to be diploid (78%). Nine grade 2 carcinomas (n = 12) exhibited aneuploidy (75%), as did all grade 3 and in situ carcinomas (n = 10). Multiploidy was demonstrated from imprints in four cases instead of the two detected from dewaxed tissue. In 27 cases (87%), G0/G1 peaks obtained from paraffin blocks showed a shift to the left. In five cases (16%), variations in the DNA index were responsible for discrepancies in the DNA ploidy evaluation between fresh imprints and dewaxed samples of the same tumors.
Image cytometry on Feulgen-stained imprints of bladder biopsies is a simple and reliable procedure for assessing DNA ploidy in urothelial carcinomas, providing great sensitivity for detecting small aneuploid peaks and multiploid tumors. DNA image analysis of touch preparations is especially useful for carcinoma in situ and small biopsies unsuitable for Hedley's technique.
比较通过图像细胞术测量膀胱癌触片与福尔马林固定、石蜡包埋样本的DNA含量。
选择31例尿路上皮癌活检样本进行前瞻性研究。对新鲜标本制作印片。通过Hedley方法从脱蜡样本中获得细胞悬液。7微米厚的切片用于原位癌和小活检样本。使用图像细胞仪在Feulgen染色的玻片上测量DNA倍体指数。
印片分析显示，7例1级癌（n = 9）为二倍体（78%）。9例2级癌（n = 12）表现为非整倍体（75%），所有3级癌和原位癌（n = 10）也是如此。4例印片显示多倍体，而脱蜡组织仅检测到2例。27例（87%）石蜡块获得的G0/G1峰向左偏移。5例（16%）中，DNA指数的变化导致同一肿瘤新鲜印片与脱蜡样本之间DNA倍体评估出现差异。
对膀胱活检样本的Feulgen染色印片进行图像细胞术是评估尿路上皮癌DNA倍体的一种简单可靠的方法，对检测小的非整倍体峰和多倍体肿瘤具有很高的敏感性。触摸标本的DNA图像分析对原位癌和不适用于Hedley技术的小活检样本特别有用。
