Ren N, Song Y C, Bi X Z, Ding Y, Liu L H
Department of Genetics, College of Life Sciences, Wuhan University, Wuhan Hubei, People's Republic of China.
Hereditas. 1997;126(3):211-7. doi: 10.1111/j.1601-5223.1997.00211.x.
A biotin-labelling in situ hybridization technique was first used to physically map two single copy genes, cdc2 and prh1, in maize. These two genes are metabolically interrelated genes. The full-length cDNA clones cdc2ZmA and ZmPPI of genes cdc2 and prh1 were adopted as the probes. They are 1.3 and 1.6 kb in size, respectively. Clone cdc2ZmA was physically mapped on the long arm of chromosomes 4, 8, and 9. The percent distances from centromere to detection site were 57.9 +/- 2.7, 28.4 +/- 1.5, and 88.2 +/- 3.3. The detection rate was 19.2%. Clone ZmPPI was physically mapped on the long arm of chromosomes 4, 6, and 8. The percent distances were 53.6 +/- 1.2, 60.8 +/- 2.9 and 17.1 +/- 1.6. The detection ratio was 18.5%. The technique of chromosome ISH and the relationship between the location and function of these two genes have been discussed.
一种生物素标记原位杂交技术首次用于对玉米中的两个单拷贝基因cdc2和prh1进行物理定位。这两个基因是代谢相关基因。采用基因cdc2和prh1的全长cDNA克隆cdc2ZmA和ZmPPI作为探针。它们的大小分别为1.3和1.6 kb。克隆cdc2ZmA被物理定位在第4、8和9号染色体的长臂上。从着丝粒到检测位点的百分比距离分别为57.9±2.7、28.4±1.5和88.2±3.3。检测率为19.2%。克隆ZmPPI被物理定位在第4、6和8号染色体的长臂上。百分比距离分别为53.6±1.2、60.8±2.9和17.1±1.6。检测率为18.5%。本文讨论了染色体原位杂交技术以及这两个基因的位置与功能之间的关系。
