Malaquias L C, Falcão P L, Silveira A M, Gazzinelli G, Prata A, Coffman R L, Pizziolo V, Souza C P, Colley D G, Correa-Oliveira R
Centro de Pesquisas René Rachou-FIOCRUZ, Belo Horizonte, Brazil.
Scand J Immunol. 1997 Oct;46(4):393-8. doi: 10.1046/j.1365-3083.1997.d01-136.x.
The role of cytokines on the in vitro proliferative response of peripheral blood mononuclear cells (PBMC) from Schistosoma mansoni infected patients to soluble egg (SEA) and adult worm antigens (SWAP) were evaluated. The results obtained demonstrated that the proliferative response of PBMC from chronic intestinal (INT) patients to SEA and SWAP is increased by the blockage of IL-10 with specific monoclonal antibodies (MAb). The effects of these antibodies were readily reversed by the addition of recombinant IL-10. In contrast, no effect was observed on the PBMC response of acute and hepatosplenic patients (HS) in the presence of anti-IL-10. Anti-IL-4 antibodies decreased the PBMC response of the intestinal (INT) and HS individuals to SEA and SWAP, and the PBMC response of acute patients to SEA but not to SWAP. Addition of anti-IL-5 MAb did not decrease the PBMC response of acute patients to SEA or SWAP. These results suggested that IL-10 has an important role in the modulation of the immune response in chronic asymptomatic patients and that this cytokine may be an important factor in controlling morbidity.
评估了细胞因子对曼氏血吸虫感染患者外周血单个核细胞（PBMC）体外增殖反应的影响，该反应针对可溶性虫卵抗原（SEA）和成虫抗原（SWAP）。所得结果表明，用特异性单克隆抗体（MAb）阻断IL-10可增强慢性肠道（INT）患者PBMC对SEA和SWAP的增殖反应。添加重组IL-10可轻易逆转这些抗体的作用。相反，在存在抗IL-10的情况下，未观察到对急性和肝脾型患者（HS）PBMC反应的影响。抗IL-4抗体降低了肠道（INT）和HS个体PBMC对SEA和SWAP的反应，以及急性患者PBMC对SEA而非SWAP的反应。添加抗IL-5 MAb并未降低急性患者PBMC对SEA或SWAP的反应。这些结果表明，IL-10在慢性无症状患者免疫反应的调节中起重要作用，并且这种细胞因子可能是控制发病率的重要因素。
