Dousset N, Ferretti G, Galeazzi T, Taus M, Gouaze V, Berthon G, Curatola G
INSERM U305, Equipe Bioréactifs: Spéciation et Biodisponibilite, Université Paul Sabatier, Toulouse, France.
Free Radic Res. 1997 Sep;27(3):291-9. doi: 10.3109/10715769709065767.
We report here an investigation of the influence of aluminium on iron-induced peroxidation in brain model membranes. Laurdan fluorescence emission spectra and generalised polarisation measurements have been used to investigate how ferrous and aluminium ions can affect the phase components of phospholipid membranes. An increase in the generalised polarisation of oxidised liposomes with respect to controls has been observed, which reveals the presence of a less polar environment surrounding the probe that changes the properties of the bilayer. Aluminium has been shown to facilitate iron-mediated oxidation as detected from emission fluorescence spectra. However, no quantitative influence has been calculated relative to general polarisation and derived phase state determinations. The structural influence of aluminium on membranes may therefore be less significantly marked than initially expected.
我们在此报告一项关于铝对脑模型膜中铁诱导的过氧化作用影响的研究。劳丹荧光发射光谱和广义极化测量已被用于研究亚铁离子和铝离子如何影响磷脂膜的相成分。已观察到氧化脂质体相对于对照的广义极化增加，这揭示了探针周围存在极性较小的环境，该环境改变了双层膜的性质。从发射荧光光谱检测到铝已被证明可促进铁介导的氧化。然而，相对于广义极化和推导的相态测定，尚未计算出定量影响。因此，铝对膜的结构影响可能没有最初预期的那么显著。
