腐败希瓦氏菌MR-1的延胡索酸还原酶缺陷型转座子突变体的分离与鉴定

Isolation and characterization of a transposon mutant of Shewanella putrefaciens MR-1 deficient in fumarate reductase.

作者信息

Myers C R, Myers J M

机构信息

Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA.

出版信息

Lett Appl Microbiol. 1997 Sep;25(3):162-8. doi: 10.1046/j.1472-765x.1997.00196.x.

DOI:10.1046/j.1472-765x.1997.00196.x

PMID:9351256

Abstract

A transposon mutant, designated CMTn-3, of Shewanella putrefaciens MR-1 that was deficient in fumarate reduction was isolated and characterized. In contrast to the wild-type, CMTn-3 could not grow anaerobically with fumarate as the electron acceptor, and it lacked benzyl viologen-linked fumarate reductase activity. Consistent with this, CMTn-3 lacked a 65 kDa c-type cytochrome, which is the same size as the fumarate reductase enzyme. CMTn-3 retained the wild-type ability to use nitrate, iron(III), manganese(IV) and trimethylamine N-oxide (TMAO) as terminal electron acceptors. The results indicate that the loss of the fumarate reductase enzyme does not affect other anaerobic electron transport systems in this bacterium.

摘要

分离并鉴定了腐败希瓦氏菌MR-1的一个转座子突变体，命名为CMTn-3，该突变体缺乏延胡索酸还原能力。与野生型相比，CMTn-3不能以延胡索酸作为电子受体进行厌氧生长，并且缺乏与苄基紫精相连的延胡索酸还原酶活性。与此一致的是，CMTn-3缺乏一种65 kDa的c型细胞色素，其大小与延胡索酸还原酶相同。CMTn-3保留了以硝酸盐、铁（III）、锰（IV）和三甲胺N-氧化物（TMAO）作为末端电子受体的野生型能力。结果表明，延胡索酸还原酶的缺失并不影响该细菌中的其他厌氧电子传递系统。