胰岛素样生长因子（IGFs）及其受体的表达是胚胎质量的一个潜在标志物。
Expression of IGFs and their receptors is a potential marker for embryo quality.
作者信息
Liu H C, He Z Y, Mele C A, Veeck L L, Davis O K, Rosenwaks Z
机构信息
Center for Reproductive Medicine and Infertility, Cornell University Medical College, New York, New York, USA.
出版信息
Am J Reprod Immunol. 1997 Oct;38(4):237-45. doi: 10.1111/j.1600-0897.1997.tb00509.x.
PROBLEM
Insulin-like growth factors (IGFs) and insulin have been demonstrated to stimulate oocyte maturation and embryo development. Therefore, the expression of IGFs and their receptors may be an important intrinsic factor for embryo growth and may be a potential marker for embryo quality.
METHOD OF STUDY
Thirty donated day 3 embryos were cultured in vitro for an additional 3 days to observe their developmental potential and were semiquantitatively analyzed for the expression of IGF-I, IGF-II, IGF-IR, IGF-IIR, and insulin-R.
RESULTS
Our results show that the activity of these gene expressions correlates well with the morphological assessment and that high and more gene expressions were often associated with embryos of high growth potential.
CONCLUSION
The IGF system may indeed play an important role in human embryogenesis; IGF gene expressions can be a good indicator of embryonic developmental stage and/or growth potential; finally, the IGF system can serve as a marker for embryo quality.
问题
胰岛素样生长因子（IGFs）和胰岛素已被证明能刺激卵母细胞成熟和胚胎发育。因此，IGFs及其受体的表达可能是胚胎生长的一个重要内在因素，并且可能是胚胎质量的一个潜在标志物。
研究方法
30个捐赠的第3天胚胎在体外再培养3天，以观察其发育潜能，并对IGF-I、IGF-II、IGF-IR、IGF-IIR和胰岛素-R的表达进行半定量分析。
结果
我们的结果表明，这些基因表达的活性与形态学评估密切相关，高表达和更多的基因表达通常与具有高生长潜能的胚胎相关。
结论
IGF系统可能确实在人类胚胎发生中起重要作用；IGF基因表达可以是胚胎发育阶段和/或生长潜能的良好指标；最后，IGF系统可作为胚胎质量的标志物。
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