Wolf E, Wagner J P, Black I B, DiCicco-Bloom E
Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
Brain Res Dev Brain Res. 1997 Sep 20;102(2):305-8. doi: 10.1016/s0165-3806(97)00111-9.
Neuronal birth and neurite outgrowth have been regarded as discrete, sequential stages of development. However, we recently found that sympathetic neuroblasts often elaborate axons before mitosis, in culture [E. Wolf, I.B. Black, E. DiCicco-Bloom, Mitotic neuroblasts determine neuritic patterning of progeny, J. Comp. Neurol. 367 (1996) 623-635] and in vivo [E. Wolf, I.B. Black, E. DiCicco-Bloom, Central and peripheral neuroblasts elaborate neurites prior to division in vivo and in vitro, Soc. Neurosci. Abstr. 21 (1995) 785; E. Wolf, I.B. Black, E. DiCicco-Bloom, Mitotic neuroblasts engage in axonal outgrowth and pathfinding in vivo, Soc. Neurosci. Abstr. 22 (1996) 525]. Here, we report that cerebellar granule cells often divide with heritable neurites in vitro. Therefore, mitotic CNS precursors, in addition to peripheral neuroblasts, simultaneously undergo proliferation and process formation. Potentially, neurites on dividing precursors may allow target fields to influence directly the course of neurogenesis.
神经元的诞生和神经突的生长一直被视为发育过程中离散的、相继的阶段。然而，我们最近发现，在培养物中[E. 沃尔夫、I.B. 布莱克、E. 迪西科 - 布鲁姆，有丝分裂的神经母细胞决定子代的神经突模式，《比较神经学杂志》367 (1996) 623 - 635]以及在体内[E. 沃尔夫、I.B. 布莱克、E. 迪西科 - 布鲁姆，中枢和外周神经母细胞在体内和体外分裂前形成神经突，《神经科学学会摘要》21 (1995) 785；E. 沃尔夫、I.B. 布莱克、E. 迪西科 - 布鲁姆，有丝分裂的神经母细胞在体内进行轴突生长和路径寻找，《神经科学学会摘要》22 (1996) 525]，交感神经母细胞常常在有丝分裂之前就形成轴突。在此，我们报告小脑颗粒细胞在体外常常带着可遗传的神经突进行分裂。因此，除了外周神经母细胞外，有丝分裂的中枢神经系统前体细胞同时进行增殖和突起形成。潜在地，正在分裂的前体细胞上的神经突可能使靶区域直接影响神经发生的进程。
