Telemenakis I, Benseler F, Stenius K, Südhof T C, Brose N
Abteilung Molekulare Neurobiologie, Max-Planck-Institut für experimentelle Medizin, Göttingen/Germany.
Eur J Cell Biol. 1997 Oct;74(2):143-9.
Mutations in the Saccharomyces cerevisiae sec7 locus lead to a pleiotropic secretory phenotype that is characterized by an accumulation of Golgi cisternae and a loss of secretory granules. This indicates that the corresponding gene product sec7p is involved in the budding of secretory granules from the Golgi apparatus. Here we report the primary structure of three rat homologues of sec7p, called msec7-1, -2, and -3. The mRNAs of these genes are expressed in all tissues tested. All msec7s share the same domain structure in which an N-terminal coiled-coil domain is followed by a sec7-homology domain and a pleckstrin-homology domain. On the protein level, msec7s are present in all rat tissues tested, with highest protein levels in brain and adrenal. In the adult rat brain, they are present in soluble and membrane-associated pools.
酿酒酵母sec7位点的突变会导致多效性分泌表型，其特征是高尔基体潴泡的积累和分泌颗粒的丧失。这表明相应的基因产物sec7p参与了从高尔基体出芽形成分泌颗粒的过程。在此，我们报告了sec7p的三个大鼠同源物的一级结构，分别称为msec7-1、-2和-3。这些基因的mRNA在所有测试组织中均有表达。所有的msec7s都具有相同的结构域结构，其中N端卷曲螺旋结构域之后是sec7同源结构域和普列克底物蛋白同源结构域。在蛋白质水平上，msec7s存在于所有测试的大鼠组织中，在脑和肾上腺中的蛋白质水平最高。在成年大鼠脑中，它们存在于可溶性和膜相关组分中。
