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一种高致瘤性人二倍体子宫内膜癌细胞系的建立与鉴定

Establishment and characterization of a highly tumorigenic human diploid endometrial cancer cell line.

作者信息

Kim J W, Lee C G, Choi S K, Kim J H, Kim T E, Lee J M, Rha J G, Namkoong S E

机构信息

Department of Obstetrics and Gynecology, Kangnam St. Mary's Hospital, Catholic University Medical College, Seoul, South Korea.

出版信息

Cancer Genet Cytogenet. 1997 Nov;99(1):1-10. doi: 10.1016/s0165-4608(96)00389-5.

Abstract

A new cell line designated CUME-1 has been established from a poorly differentiated endometrial adenocarcinoma of the uterus. This cell line grew well without interruption for more than 88 months and 110 serial passages were successively carried out. The cells were highly tumorigenic in nude mice (85%). Repeated karyotype analyses from early (4th) to late (55th) passages of this cell line revealed a diploid stable clone in each passages without any noticeable structural or numerical aberrations. But from the 80th passage, a subpopulation with reciprocal translocation between chromosomes 1q and 9q consistently appeared and was observed in about 30% of the cells. This cell line is one of the rare examples of experimentally proved tumorigenic cells of human solid tumor origin that retains the diploid karyotype in vitro. HLA typing indicated the presence of DR4, DR13, DQ3 and DQ6. Cytosol estrogen and progesterone receptors were found both in fresh primary tumor and in this cell line. Gonadotropin-releasing hormone (Gn-RH) receptor mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in cultured cells. Using the single-strand conformation polymorphism (SSCP) technique, we have screened CUME-1 cells for p53 mutation in exons 4 to 9. No mobility shift was observed. This cell line may be useful in studying the in vitro chromosomal evolution of the cell line and the in vivo properties of human endometrial adenocarcinoma.

摘要

一种名为CUME-1的新细胞系已从子宫低分化子宫内膜腺癌中建立。该细胞系生长良好,连续88个月无间断,并连续传代110次。这些细胞在裸鼠中具有高度致瘤性(85%)。对该细胞系从早期(第4代)到晚期(第55代)进行的反复核型分析显示,每一代都有一个二倍体稳定克隆,没有任何明显的结构或数量异常。但从第80代开始,在1号染色体长臂和9号染色体长臂之间发生相互易位的亚群持续出现,约30%的细胞中可观察到。该细胞系是经实验证明的人类实体瘤来源的致瘤细胞中罕见的例子之一,其在体外保留二倍体核型。HLA分型显示存在DR4、DR13、DQ3和DQ6。在新鲜原发性肿瘤和该细胞系中均发现了胞质雌激素和孕激素受体。通过逆转录-聚合酶链反应(RT-PCR)在培养细胞中检测到促性腺激素释放激素(Gn-RH)受体mRNA。使用单链构象多态性(SSCP)技术,我们筛选了CUME-1细胞外显子4至9中的p53突变。未观察到迁移率改变。该细胞系可能有助于研究细胞系的体外染色体进化以及人类子宫内膜腺癌的体内特性。

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