Kim J W, Lee C G, Lyu M S, Kim H K, Rha J G, Kim D H, Kim S J, Namkoong S E
Department of Obstetrics and Gynecology, Kangnam St. Mary's Hospital, Catholic University Medical College, Seoul, Korea.
J Cancer Res Clin Oncol. 1997;123(2):82-90. doi: 10.1007/BF01269885.
A cell line designated CUMO-2 has been established from an undifferentiated ovarian carcinoma. The s.c. injection of cells into nude mice gave rise to fast-growing tumors, while the i.p. route induced a peritoneal carcinomatosis with ascites. Histopathologically, the transplanted s.c. tumors closely resembled the original tumor, but tumors developed in the peritoneal cavity were highly anaplastic. The epithelial nature of the cells was confirmed by ultrastructural analysis. Sequential cytogenetic analyses on early and late passages revealed highly aneuploid tumor cells with consistent structural aberrations of chromosomes 1, 3, 8 and 11. CUMO-2 cells were found to produce CA 125 in vitro and in vivo. Cytosol estrogen receptor (ER) was found but progesterone receptor (PR) was not measured. HLA typing indicated the presence of DR8 and DQw4. A gonadotropin-releasing hormone (Gn-RH) analog inhibited cell growth and Gn-RH receptor mRNA was detected by reverse transcription/polymerase chain reaction in this cell line. Administration of transforming growth factor beta 1 inhibited both cell growth and c-myc mRNA expression. This cell line demonstrated a conformational band shift in exon 7 of the p53 gene. It was a frameshift mutation.
一种名为CUMO-2的细胞系是从未分化的卵巢癌中建立起来的。将细胞皮下注射到裸鼠体内会产生快速生长的肿瘤，而腹腔注射则会诱发伴有腹水的腹膜癌。从组织病理学上看，移植的皮下肿瘤与原发肿瘤非常相似，但在腹腔内形成的肿瘤则高度间变。通过超微结构分析证实了细胞的上皮性质。对早期和晚期传代细胞进行的连续细胞遗传学分析显示，肿瘤细胞高度非整倍体，伴有1号、3号、8号和11号染色体一致的结构畸变。发现CUMO-2细胞在体内外均能产生CA 125。检测到细胞溶质雌激素受体（ER），但未检测孕酮受体（PR）。HLA分型显示存在DR8和DQw4。促性腺激素释放激素（Gn-RH）类似物可抑制细胞生长，并且通过逆转录/聚合酶链反应在该细胞系中检测到Gn-RH受体mRNA。给予转化生长因子β1可同时抑制细胞生长和c-myc mRNA表达。该细胞系在p53基因第7外显子中表现出构象带移。这是一种移码突变。
