Lundstrom K
F Hoffmann-La Roche, Research Laboratories, Roche Ltd, Basel, Switzerland.
Curr Opin Biotechnol. 1997 Oct;8(5):578-82. doi: 10.1016/s0958-1669(97)80032-8.
Alphavirus vectors have been used for efficient high-level expression of a variety of topologically different proteins, allowing studies of protein transport, localization and functional activity in a broad range of host cells. Complex transmembrane proteins have been produced in large quantities through the establishment of scale-up technology. Alphavirus vectors have also shown promising potential in vaccine production and preliminary gene therapy applications.
甲病毒载体已被用于高效高水平表达多种拓扑结构不同的蛋白质，从而能够在多种宿主细胞中研究蛋白质转运、定位及功能活性。通过扩大规模技术的建立，已大量生产出复杂的跨膜蛋白。甲病毒载体在疫苗生产和初步基因治疗应用中也显示出了有前景的潜力。
