Podschun R, Ullmann U
Institut für Medizinische Mikrobiologie und Virologie, Universität Kiel.
Zentralbl Hyg Umweltmed. 1996 Feb;198(3):258-64.
To evaluate whether clinical Klebsiella isolates differ from nonclinical strains with respect to bacteriocin susceptibility patterns, a total of 452 Klebsiella pneumoniae and K. oxytoca strains isolated from different sources were examined. Bacteriocin typing was done by a modification of the scrape-and-point method, using a set of eight producer strains. 96% of the strains were typable. Forty-one different bacteriocin susceptibility patterns were observed. While two thirds of the K. oxytoca isolates belonged to only three different bacteriocin types, the K. pneumoniae strains showed a more heterogeneous distribution of patterns. No differences in pattern distribution were observed between isolates from clinical, fecal, or environmental sources. Certain bacteriocins showed a very broad spectrum of activity; e.g. 93% of all isolates were susceptible to bacteriocin type 3. The results suggest that nonclinical Klebsiella strains do not show other bacteriocin susceptibility types than clinical isolates do.
为评估临床分离的克雷伯菌与非临床菌株在细菌素敏感性模式方面是否存在差异，我们检测了从不同来源分离出的总共452株肺炎克雷伯菌和产酸克雷伯菌。细菌素分型采用改良的刮涂点样法，使用一组8株产菌菌株。96%的菌株可分型。观察到41种不同的细菌素敏感性模式。虽然三分之二的产酸克雷伯菌分离株仅属于三种不同的细菌素类型，但肺炎克雷伯菌菌株的模式分布更为不均一。临床、粪便或环境来源的分离株在模式分布上未观察到差异。某些细菌素显示出非常广泛的活性谱；例如，所有分离株中有93%对3型细菌素敏感。结果表明，非临床克雷伯菌菌株与临床分离株相比，未显示出其他细菌素敏感性类型。
