Trosić I, Brumen V, Horvat D
Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Zentralbl Hyg Umweltmed. 1997 Apr;199(6):558-67.
This study was carried out in order to assess the genotoxic effect of in vitro exposure to commercial chrysotile asbestos. V 79 cell line, known as a well-established cellular model, was used for detection of asbestos genotoxic potency. Conventional structural chromosomal aberration analysis and sister chromatid exchange (SCE) method were both used for asbestos genotoxicity assessment. Within the experimental protocol applied, V 79 cells were treated with asbestos in concentrations of 100 and 200 micrograms/ml F-10 (HAM) media during 90 days, respectively. Analysis of changes in chromosome structure as well as of cell ploidy was performed each tenth day of the experimental course, consecutively. Two hundred well spread metaphases were taken into account for chromosomal aberration analysis. Frequency of sister chromatid exchanges was observed in 50 cells per sample. The results of cytogenetic tests revealed structural chromosomal damages, SCE-elevation and changes in cell ploidy. Cytogenetic effect of asbestos obviously depended on the dose applied and on the period of incubation. The results of this study suggest that significant cytogenetic changes occurring after asbestos treatment might directly or indirectly be the part of the biological events responsible for eliciting asbestos-induced carcinogenesis.
本研究旨在评估体外暴露于商用温石棉的遗传毒性作用。V 79细胞系作为一种成熟的细胞模型，用于检测石棉的遗传毒性效力。常规的结构染色体畸变分析和姐妹染色单体交换（SCE）方法均用于石棉遗传毒性评估。在所应用的实验方案中，V 79细胞分别在90天内用浓度为100和200微克/毫升F-10（HAM）培养基的石棉处理。在实验过程的每十天连续进行染色体结构变化以及细胞倍性分析。染色体畸变分析考虑了200个分散良好的中期细胞。每个样品在50个细胞中观察姐妹染色单体交换频率。细胞遗传学测试结果显示出染色体结构损伤、SCE升高和细胞倍性变化。石棉的细胞遗传学效应明显取决于所用剂量和孵育时间。本研究结果表明，石棉处理后发生的显著细胞遗传学变化可能直接或间接地是引发石棉诱导致癌作用的生物学事件的一部分。
