Hammond J, Dienelt M M
United States Department of Agriculture, Agricultural Research Service, United States National Arboretum, Beltsville, MD 20705, USA.
Mol Plant Microbe Interact. 1997 Nov;10(8):1023-7. doi: 10.1094/MPMI.1997.10.8.1023.
Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from < 1% to as much as 25% transgenic CP. Different levels of transcapsidation may reflect the extent of compatibility between transgene CP and the viral CP.
用各种马铃薯Y病毒接种表达菜豆黄花叶马铃薯Y病毒或嵌合马铃薯Y病毒外壳蛋白（CP）的转基因植物。对从转基因植物中纯化的病毒进行抗原包被平板、间接酶联免疫吸附测定和免疫电子显微镜分析，结果表明子代病毒粒子含有低至1%到高达25%的转基因CP。不同水平的转衣壳现象可能反映了转基因CP与病毒CP之间的相容程度。
