Chanda J, Kuribayashi R, Abe T
Department of Cardiovascular Surgery, Akita University School of Medicine, Japan.
Ann Thorac Surg. 1997 Oct;64(4):1063-6. doi: 10.1016/s0003-4975(97)00819-9.
The problem of calcification in porcine aortic (AVs) and pulmonary (PVs) valves and its relationship to glutaraldehyde (GA) is of current interest. We proposed an anticalcification treatment to develop noncalcifying porcine AVs and PVs.
Porcine AVs and PVs were cross-linked in GA. Partially degraded heparin was coupled to the GA-treated AVs and PVs through intermediate surface-bound substrate containing amino groups. Control AVs and PVs were cross-linked in 0.625% GA but had no heparin coupling. All specimens were implanted subdermally in 3-week-old rats for 5 months for calcification studies.
Control AVs (Ca, 233.69 +/- 42.61 mg/g) and PVs (Ca, 181.48 +/- 4.06 mg/g) were severely calcified. Coupling of partially degraded heparin revealed complete prevention of calcification in GA-treated AVs (Ca, 0.73 +/- 0.27) and PVs (Ca, 1.125 +/- 0.22 mg/g) implanted subcutaneously in weanling rats for 5 months.
The proposed anticalcification treatment is effective in preventing calcification of GA-treated AVs and PVs implanted subcutaneously in weanling rats for 5 months.
猪主动脉瓣（AVs）和肺动脉瓣（PVs）的钙化问题及其与戊二醛（GA）的关系是当前研究的热点。我们提出了一种抗钙化处理方法，以开发无钙化的猪AVs和PVs。
猪AVs和PVs在GA中进行交联。部分降解的肝素通过含有氨基的中间表面结合底物与经GA处理的AVs和PVs偶联。对照AVs和PVs在0.625%的GA中交联，但未进行肝素偶联。所有标本均皮下植入3周龄大鼠体内5个月，以进行钙化研究。
对照AVs（钙含量为233.69±42.61mg/g）和PVs（钙含量为181.48±4.06mg/g）严重钙化。部分降解的肝素偶联显示，在断奶大鼠皮下植入5个月的经GA处理的AVs（钙含量为0.73±0.27）和PVs（钙含量为1.125±0.22mg/g）中完全预防了钙化。
所提出的抗钙化处理方法对于预防在断奶大鼠皮下植入5个月的经GA处理的AVs和PVs的钙化是有效的。
