Moreno C, Hale C, Hewett R, Cussell D
Eur J Immunol. 1979 Nov;9(11):916-9. doi: 10.1002/eji.1830091116.
Isomaltohexaose flavazole coupled to chicken gamma-globulin (IM6-CGG) induced T cell-dependent anti-alpha(1 leads to 6) dextran-specific IgM and IgG responses in CBA, BALB/c and A strain mice. The IgG responses were of restricted heterogeneity as judged by isoelectric focusing, and belonged mostly to the IgG1 subclass with a minor IgG3 component in the case of BALB/c and CBA mice. All four subclasses of IgG were produced in A strain mice. In contrast, native dextran B 512 induces exclusively T cell-independent IgM responses of the same specificity for all 3 strains. All BALB/c mice immunized with different doses of IM6-CGG either in Freund's adjuvant or with Al(OH)3 plus Bordetella pertussis showed the same spectrotypes by isoelectric focusing. Sera obtained at different times after immunization of BALB/c mice failed to show spectrotype variations. Late, but not early, bleedings from CBA mice showed a tendency towards more uniform isoelectric focusing patterns.
异麦芽六糖黄酮唑偶联鸡γ球蛋白（IM6-CGG）在CBA、BALB/c和A品系小鼠中诱导了T细胞依赖性抗α(1→6)葡聚糖特异性IgM和IgG反应。通过等电聚焦判断，IgG反应具有有限的异质性，在BALB/c和CBA小鼠中，主要属于IgG1亚类，有少量IgG3成分。A品系小鼠产生了所有四种IgG亚类。相比之下，天然葡聚糖B 512对所有这3个品系仅诱导相同特异性的T细胞非依赖性IgM反应。所有用不同剂量的IM6-CGG在弗氏佐剂中或与氢氧化铝加百日咳博德特氏菌一起免疫的BALB/c小鼠，通过等电聚焦显示出相同的光谱类型。在BALB/c小鼠免疫后不同时间获得的血清未显示光谱类型变化。CBA小鼠较晚而非较早的采血显示出等电聚焦模式更趋一致的趋势。
