Adhirai M, Selvam R
Department of Biochemistry, Dr. A.L.M. PG Institute of Basic Medical Sciences, University of Madras, Taramani, India.
Jpn J Med Sci Biol. 1997 Feb;50(1):9-17. doi: 10.7883/yoken1952.50.9.
The effect of cyclosporin A, a highly effective immunosuppressant, was investigated on hyperoxaluric rats with and without vitamin E pretreatment. Hyperoxaluria was induced by oral feeding of 3% ammonium oxalate in water for 3 days. Cyclosporin A (50 mg/kg body wt.) was administered for 3 days. Pretreatment with vitamin E (50 mg/100 g body wt., once a week for 3 weeks) was carried out before the administration of cyclosporin A and ammonium oxalate. Nonenzymatic ascorbate-induced lipid peroxidation was increased to 1.55-fold in either cyclosporin A-administered or hyperoxaluric rat kidney and liver when compared to control. The lipid peroxidation was further elevated to 1.9-fold when both cyclosporin A and ammonium oxalate were coadministered. The activities of renal and hepatic ATPase, glucose-6-phosphatase as well as the concentrations of thiols were decreased significantly (p < 0.001) when cyclosporin A was administered under hyperoxaluric condition. On pretreatment with vitamin E the cyclosporin A-induced biochemical changes observed in the presence of hyperoxaluria were abolished.
研究了高效免疫抑制剂环孢素A对有或无维生素E预处理的高草酸尿大鼠的影响。通过在水中口服3%草酸铵3天诱导高草酸尿症。给予环孢素A（50mg/kg体重）3天。在给予环孢素A和草酸铵之前，先用维生素E（50mg/100g体重，每周一次，共3周）进行预处理。与对照组相比，在给予环孢素A的大鼠或高草酸尿大鼠的肾脏和肝脏中，非酶促抗坏血酸诱导的脂质过氧化增加到1.55倍。当同时给予环孢素A和草酸铵时，脂质过氧化进一步升高到1.9倍。在高草酸尿条件下给予环孢素A时，肾和肝ATP酶、葡萄糖-6-磷酸酶的活性以及硫醇的浓度显著降低（p<0.001）。用维生素E预处理后，在高草酸尿存在的情况下观察到的环孢素A诱导的生化变化被消除。
