Margo C E, Lowery R L, Kerschmann R L
Department of Ophthalmology, University of South Florida, College of Medicine, Tampa 33612, USA.
Retina. 1997;17(5):434-6. doi: 10.1097/00006982-199709000-00012.
Bilateral diffuse uveal melanocytic proliferation is a poorly understood disorder characterized by the progressive proliferation of uveal melanocytes associated with a systemic nonocular malignancy. Overexpression of p53 protein plays a role in the loss of regulatory control of normal cell proliferation, and p53 is the most commonly identified oncogenic protein in human malignancies. We tested the hypothesis that the aberrant cellular activity in bilateral diffuse uveal melanocytic proliferation involves the overexpression of p53 protein.
Eight eyes from four patients with bilateral diffuse uveal melanocytic proliferation were tested for p53 protein using an immunoperoxidase technique with an anti-p53 protein monoclonal antibody sensitive for normal and mutant p53 protein.
The p53 protein could not be detected in any of the eight eyes.
The proliferation of uveal melanocytes in bilateral diffuse uveal melanocytic proliferation does not depend on the overexpression of p53 protein. The loss of cellular regulatory control in bilateral diffuse uveal melanocytic proliferation is probably mediated through another mechanism.
双侧弥漫性葡萄膜黑素细胞增生是一种了解甚少的疾病，其特征为葡萄膜黑素细胞进行性增生，并伴有全身性非眼恶性肿瘤。p53蛋白的过表达在正常细胞增殖调控失控中起作用，且p53是人类恶性肿瘤中最常鉴定出的致癌蛋白。我们检验了这样一个假设，即双侧弥漫性葡萄膜黑素细胞增生中异常的细胞活性涉及p53蛋白的过表达。
使用对正常和突变型p53蛋白敏感的抗p53蛋白单克隆抗体，采用免疫过氧化物酶技术，对4例双侧弥漫性葡萄膜黑素细胞增生患者的8只眼睛进行p53蛋白检测。
在这8只眼睛中均未检测到p53蛋白。
双侧弥漫性葡萄膜黑素细胞增生中葡萄膜黑素细胞的增殖不依赖于p53蛋白的过表达。双侧弥漫性葡萄膜黑素细胞增生中细胞调控失控可能是通过另一种机制介导的。
