Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein.
作者信息
Klausen J, Pérez V, Giese S B, García Marín J F, Ahrens P
机构信息
Danish Veterinary Laboratory, Copenhagen, Denmark.
出版信息
Vet Microbiol. 1997 Sep;57(2-3):181-7. doi: 10.1016/s0378-1135(97)00133-8.
PMID:9355253
Abstract
A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.
摘要
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