Klausen J, Giese S B, Fuursted K, Ahrens P
Danish Veterinary Laboratory, Copenhagen, Denmark.
APMIS. 1997 Apr;105(4):277-82. doi: 10.1111/j.1699-0463.1997.tb00569.x.
The aim of the present study was to characterize all strains of the Mycobacterium avium complex isolated in Denmark in 1993. A total of 141 M. avium complex strains (86 from man, 38 from animals, and 17 from peat) were analysed by serotyping, ELISA specific for a 40 kDa protein, and IS901-specific PCR. Serotype analysis showed that the most frequent serotypes among human strains were serotype 4 (27%) and serotype 6 (19%), which differs from an earlier survey where serotype 1 was most prevalent. The most frequent serotypes in animals were serotype 2 (53%) and serotype 6 (13%), whereas the most prevalent serotypes among strains isolated from peat were serotype 4 (29%) and serotype 9 (18%). There was a concurrent appearance of IS901 and p40 in all strains. Only M. avium complex strains isolated from animals, and belonging to serotype 1 or serotype 2, contained the IS901/p40 markers. The different distribution of serotypes of M. avium complex strains in animals and man, and the presence of IS901/p40 exclusively in animal strains, suggests that transmission of M. avium from animals to man is not of significance in Denmark.
本研究的目的是对1993年在丹麦分离出的所有鸟分枝杆菌复合群菌株进行特征分析。通过血清分型、针对40 kDa蛋白的ELISA以及IS901特异性PCR对总共141株鸟分枝杆菌复合群菌株（86株来自人类，38株来自动物，17株来自泥炭）进行了分析。血清型分析表明，人类菌株中最常见的血清型是血清型4（27%）和血清型6（19%），这与早期血清型1最为普遍的调查结果不同。动物中最常见的血清型是血清型2（53%）和血清型6（13%），而从泥炭中分离出的菌株中最普遍的血清型是血清型4（29%）和血清型9（18%）。所有菌株中IS901和p40同时出现。仅从动物分离出的、属于血清型1或血清型2的鸟分枝杆菌复合群菌株含有IS901/p40标记。鸟分枝杆菌复合群菌株在动物和人类中的血清型分布不同，且IS901/p40仅存在于动物菌株中，这表明在丹麦，鸟分枝杆菌从动物传播给人类并不重要。
