Masters A M, Ellis T M, Grein S B
Animal Health Laboratories, Agriculture W.A, South Perth, Western Australia.
Vet Microbiol. 1997 Sep;57(2-3):199-213. doi: 10.1016/s0378-1135(97)00130-2.
Interactions between Dermatophilus congolensis strains and with other bacteria of known haemolytic activities were used to elucidate the complex nature of haemolytic activities present in various D. congolensis strains. This was further analysed by measuring their specific phospholipase activities against defined substrates by thin layer chromatography. D. congolensis strains demonstrated haemolytic interactions (synergistic or antagonistic) with other D. congolensis strains and also other species of bacteria. Most isolates expressed lyso-phospholipase-D activity, while various strains also expressed sphingomyelinase-D activity, phospholipase-A versus phosphatidylcholines and/or cephalins, phospholipase-D versus phosphatidylcholines or all these activities, under the culture conditions used.
利用刚果嗜皮菌菌株之间以及与其他已知具有溶血活性的细菌之间的相互作用，来阐明各种刚果嗜皮菌菌株中溶血活性的复杂性质。通过薄层层析法测量它们对特定底物的特异性磷脂酶活性，对此进行了进一步分析。刚果嗜皮菌菌株与其他刚果嗜皮菌菌株以及其他细菌种类表现出溶血相互作用（协同或拮抗）。在所用培养条件下，大多数分离株表达溶血磷脂酶-D活性，而各种菌株还表达鞘磷脂酶-D活性、针对磷脂酰胆碱和/或脑磷脂的磷脂酶-A、针对磷脂酰胆碱的磷脂酶-D或所有这些活性。
