Myllys V, Ridell J, Björkroth J, Biese I, Pyörälä S
National Veterinary and Food Research Institute, Helsinki, Finland.
Vet Microbiol. 1997 Sep;57(2-3):245-51. doi: 10.1016/s0378-1135(97)00137-5.
Staphylococcus aureus isolates (N = 40) from bovine mastitis were characterized by random amplified polymorphic DNA-PCR (RAPD-PCR), ribotyping and biotyping. The isolates were collected in the veterinary surveillance area of the Ambulatory Clinic, Faculty of Veterinary Medicine, University of Helsinki from 20 quarters during the acute phase of infection and from the same quarters 3 weeks after cessation of therapy. The aim of the study was to compare the S. aureus isolates taken from the same quarter at different times to verify persistence of virulent strains in infected quarters and to compare the discriminatory power of the diagnostic methods. Using all methods (except for a commercial diagnostic test), the paired isolates of S. aureus were identical. Results suggest that the chronic nature of S. aureus infections was due to the persistence of the original infective strain. More laborious ribotyping and the more convenient RAPD-PCR method produced identical results. The molecular methods differentiated the 40 isolates into 6 distinct genotypes. Biotyping produced partially identical results to RAPD and ribotyping. A commercial diagnostic test system identified only 3 S. aureus biotypes.
采用随机扩增多态性DNA-PCR（RAPD-PCR）、核糖体分型和生物分型方法，对从患乳房炎奶牛分离出的40株金黄色葡萄球菌进行了特征分析。这些菌株采自赫尔辛基大学兽医学院门诊诊所的兽医监测区域，在感染急性期从20个奶牛乳房采集，在治疗停止3周后从相同乳房再次采集。本研究的目的是比较在不同时间从同一乳房采集的金黄色葡萄球菌菌株，以验证感染乳房中致病菌株的持续性，并比较诊断方法的鉴别能力。使用所有方法（除了一种商业诊断测试），配对的金黄色葡萄球菌菌株是相同的。结果表明，金黄色葡萄球菌感染的慢性特征是由于原始感染菌株的持续存在。更繁琐的核糖体分型和更简便的RAPD-PCR方法产生了相同的结果。分子方法将40株菌株分为6个不同的基因型。生物分型产生的结果与RAPD和核糖体分型部分相同。一种商业诊断测试系统仅鉴定出3种金黄色葡萄球菌生物型。
