Ozasa T, Newton M R, Dallman M J, Shimizu S, Gray D W, Morris P J
Nuffield Department of Surgery, University of Oxford, Oxford Radcliffe Hospital, England.
Transplantation. 1997 Oct 27;64(8):1152-9. doi: 10.1097/00007890-199710270-00013.
We examined the production of cytokine message in allogeneic and syngeneic rat pancreatic islet grafts using specific primers and polymerase chain reaction. Freshly isolated islet preparations contained transcripts for interleukin (IL)-1alpha, IL-6, IL-10, and interferon-gamma (IFN-gamma) but not for IL-2. IL-1alpha in allogeneic grafts showed increased and consistently high expression from 1 to 7 days after transplantation, but the level in syngeneic grafts fell quickly to pretransplant levels. IL-2 and IFN-gamma transcripts were found in allogeneic grafts at 1, 3, 5, and 7 days after transplantation with a peak at day 5, but these cytokines were almost absent from syngeneic grafts. The peak of IL-6 expression was 1 day after transplantation in both syngeneic and allogeneic grafts, and then the level fell quickly. IL-10 was produced at approximately the same high level at all time points in both syngeneic and allogeneic grafts. The results show that freshly isolated islet preparations contain IL-1alpha, IL-6, IL-10, and IFN-gamma transcripts at the time of transplantation. The initial production of cytokines in islet grafts, especially IL-1, may explain phenomena such as graft nonfunction, rapid rejection, and lack of response to immunosuppression.
我们使用特异性引物和聚合酶链反应检测了同种异体和同基因大鼠胰岛移植中细胞因子信使的产生。新鲜分离的胰岛制剂含有白细胞介素（IL）-1α、IL-6、IL-10和干扰素-γ（IFN-γ）的转录本，但不含IL-2的转录本。同种异体移植中的IL-1α在移植后1至7天表达增加且持续处于高水平，但同基因移植中的水平迅速降至移植前水平。在移植后1、3、5和7天的同种异体移植中发现了IL-2和IFN-γ转录本，在第5天达到峰值，但同基因移植中几乎没有这些细胞因子。IL-6表达的峰值在同基因和同种异体移植中均在移植后1天出现，然后水平迅速下降。IL-10在同基因和同种异体移植的所有时间点产生的水平大致相同。结果表明，新鲜分离的胰岛制剂在移植时含有IL-1α、IL-6、IL-10和IFN-γ转录本。胰岛移植中细胞因子的初始产生，尤其是IL-1，可能解释移植物无功能、快速排斥和对免疫抑制无反应等现象。
