Lillo O L, Severgnini A A, Nunes E M
Department of Biophysics, Faculty of Medicine, Montevideo, Uruguay.
Radiat Res. 1997 Nov;148(5):476-80.
The mutagenic interactions of ultraviolet light and bleomycin in haploid populations of Saccharomyces cerevisiae were analyzed. Survival and mutation frequency as a function of different bleomycin concentrations after one conditioning dose of UV radiation were determined. Furthermore, corresponding interaction functions and sensitization factors were calculated. A synergistic interaction between UV light and bleomycin was shown for both lethal and mutagenic events when the cells were in nutrient broth during the treatments. Conversely, the interaction between UV light and bleomycin was antagonistic when the cells were in deionized water during the treatment. The magnitude of lethal and mutagenic interactions depends on dose, and thus presumably on the number of lesions. The observed interactions between UV light and bleomycin suggest that the mechanism that is most likely involved is the induction of repair systems with different error probabilities during the delay of cell division.
分析了紫外线和博来霉素在酿酒酵母单倍体群体中的诱变相互作用。测定了在一次紫外线辐射预处理剂量后，作为不同博来霉素浓度函数的存活率和突变频率。此外，还计算了相应的相互作用函数和致敏因子。当处理过程中细胞处于营养肉汤中时，紫外线和博来霉素之间对致死和诱变事件均表现出协同相互作用。相反，当处理过程中细胞处于去离子水中时，紫外线和博来霉素之间的相互作用是拮抗的。致死和诱变相互作用的程度取决于剂量，因此大概取决于损伤的数量。观察到的紫外线和博来霉素之间的相互作用表明，最可能涉及的机制是在细胞分裂延迟期间诱导具有不同错误概率的修复系统。
