Bringmann G, Mader T, Feineis D
Institute of Organic Chemistry, University of Würzburg, Am Hubland, Würzburg, D-97074, Germany.
Anal Biochem. 1997 Nov 1;253(1):18-25. doi: 10.1006/abio.1997.2362.
A sensitive gas chromatographic method for the determination of the quinoline alkaloid viridicatin, a fungal metabolite isolated from several Penicillium species and presumably a biogenetic precursor to naturally occurring diazepam-like 1,4-benzo-diazepines, has been developed. Prior to conversion of viridicatin into a volatile trimethylsilyl derivative, a rapid sample clean-up was accomplished by solid-phase extraction from mould mycelium on a C18 cartridge. Using synthetical 6-bromo-viridicatin as an internal standard, the amount of viridicatin produced de novo in emerged grown cultures of Penicillium cyclopium strain SM 72 was calculated in correlation to the duration of mould cultivation.
已开发出一种灵敏的气相色谱法，用于测定喹啉生物碱绿青霉素，它是从几种青霉菌中分离出的一种真菌代谢产物，据推测是天然存在的类似地西泮的1,4-苯并二氮杂卓的生物合成前体。在将绿青霉素转化为挥发性三甲基硅烷基衍生物之前，通过在C18柱上从霉菌菌丝体进行固相萃取完成了快速样品净化。使用合成的6-溴绿青霉素作为内标，计算了环青霉菌株SM 72的新生培养物中从头产生的绿青霉素量与霉菌培养持续时间的相关性。
