γ-氨基丁酸（GABA）和琥珀酸半醛对种子萌发及某些酶活性的保护作用，使其免受高浓度羟胺的影响。

Protection by GABA and succinic semialdehyde of seed germination and some enzymatic activities against high concentration of hydroxylamine.

作者信息

Cañadas S, González M P, Ventura M E

出版信息

Rev Esp Fisiol. 1976 Jun;32(2):91-4.

Abstract

Hydroxylamine was found to stimulate germination of Lupinus albus at concentrations inferior to 10 mM and to inhibit it greatly at 20 mM concentration. This inhibition was partially restored by GABA or succinic semialdehyde. Hydroxylamine, at high concentrations, behaved as inhibitor in vivo on GABA 2-oxyglutarate amino-transferase and succinic semialdehyde dehydrogenase NAD-dependent, whereas it behaved as activator on succinic semialdehyde dehydrogenase NADP-dependent. No effects were observed on the enzymatic activities and the inhibited germination was partially restored, after GABA and succinic semialdehyde had been added to a growth medium with a 20 mM hydroxylamine concentration. A possible protection mechanism of GABA and succinic semialdehyde against hydroxylamine action is discussed.

摘要

研究发现，羟胺在浓度低于10 mM时可刺激白羽扇豆发芽，而在20 mM浓度时则会极大地抑制其发芽。γ-氨基丁酸（GABA）或琥珀酸半醛可部分恢复这种抑制作用。高浓度的羟胺在体内对依赖NAD的GABA 2-氧代戊二酸氨基转移酶和琥珀酸半醛脱氢酶起抑制作用，而对依赖NADP的琥珀酸半醛脱氢酶则起激活作用。在含有20 mM羟胺的生长培养基中添加GABA和琥珀酸半醛后，未观察到对酶活性的影响，且受抑制的发芽部分得到恢复。本文讨论了GABA和琥珀酸半醛对羟胺作用的一种可能的保护机制。