Nakamura I, Saito M, Fukumoto Y, Yoshida M, Nishi K, Weiss R M, Latifpour J
Section of Urology, Yale University School of Medicine, New Haven, CT 06520, USA.
Peptides. 1997;18(7):1091-3. doi: 10.1016/s0196-9781(97)00026-0.
We investigated the binding characteristics of endothelin (ET) receptors in the ureters of rats with experimentally induced diabetes and diuresis. Receptor binding experiments demonstrated an upregulation in the expression of [125I]ET-1 binding sites in the diabetic rat ureter but not in the diuretic rat ureter. ET-1, ET-3, IRL 1620, and BQ 610 inhibited [125I]ET-binding to the rat ureter consistent with the predominance of ETA receptors in these tissues. The subtype specificity of ET receptors in ureteral tissues was confirmed with inhibition data obtained from cloned human ETA and ETB receptors.
我们研究了实验性诱导糖尿病和利尿大鼠输尿管中内皮素（ET）受体的结合特性。受体结合实验表明，糖尿病大鼠输尿管中[125I]ET-1结合位点的表达上调，而利尿大鼠输尿管中未上调。ET-1、ET-3、IRL 1620和BQ 610抑制[125I]ET与大鼠输尿管的结合，这与这些组织中ETA受体占主导地位一致。通过从克隆的人ETA和ETB受体获得的抑制数据，证实了输尿管组织中ET受体的亚型特异性。
