Abramsson-Zetterberg L, Grawé J, Zetterberg G
Department of Genetics, Uppsala University, Sweden.
Mutat Res. 1997 Sep 18;393(1-2):55-71. doi: 10.1016/s1383-5718(97)00086-7.
Almost 100 animals of 4 different species of small wild rodents (bank vole, Clethrionomys glareolus; field vole, Microtus agrestis; yellow-necked mouse, Apodemus flavicollis; and wood mouse, Apodemus sylvaticus) were trapped in central Sweden and used in experiments to determine the spontaneous and radiation-induced frequencies of polychromatic (fMPCE) and normochromatic erythrocytes (fMNCE) from bone marrow (bm) and peripheral blood (pb) using flow cytometric analysis. The results were compared with those from similar experiments with CBA mice. The saving of time and labour by the use of the flow cytometer-based analysis was a prerequisite for this study in which about 135 million PCE were analysed. The two species of voles had a mean background fMPCE (bm) of about the same value as CBA mice, while the yellow-necked mice had about five times higher fMPCE (bm). Wood mice had more than twice the fMPCE (bm) compared to CBA mice. Between individual animals in each of the 4 species, the background fMPCE (bm) varied more than between individual CBA mice, and the elimination of micronucleated erythrocytes was considerable. When exposed to ionizing radiation, the voles did not show a significant response. The response of the two Apodemus species was similar to that of the CBA mice, although it varied between individual animals and was not correlated to their background fMPCE. This study indicates that bank voles and field voles are unsuitable testing objects in the in vivo micronucleus assay. On the other hand, yellow-necked mice and wood mice seem to be useful in this test. Since the variation between individuals is considerable in wild Apodemus mice, large groups will be needed for obtaining statistically significant results when exposure to a genotoxic agent is low. Alternatively, repeated samples can be taken from individual wild mice to study the effect of a decreased exposure after keeping the animals for a period of time in an uncontaminated environment.
在瑞典中部捕获了近100只4种不同小型野生啮齿动物（棕背䶄，Clethrionomys glareolus；田鼠，Microtus agrestis；黄颈姬鼠，Apodemus flavicollis；和林姬鼠，Apodemus sylvaticus），并用于实验，通过流式细胞术分析来确定骨髓（bm）和外周血（pb）中多色红细胞（fMPCE）和正色红细胞（fMNCE）的自发频率和辐射诱导频率。将结果与CBA小鼠的类似实验结果进行比较。使用基于流式细胞仪的分析节省时间和劳动力是本研究的一个前提条件，本研究分析了约1.35亿个多色红细胞。两种田鼠的平均背景fMPCE（bm）与CBA小鼠的值大致相同，而黄颈姬鼠的fMPCE（bm）约高五倍。林姬鼠的fMPCE（bm）是CBA小鼠的两倍多。在这4个物种的每只动物个体之间，背景fMPCE（bm）的差异比CBA小鼠个体之间的差异更大，并且微核红细胞的消除相当可观。当暴露于电离辐射时，田鼠没有显示出显著反应。两种姬鼠的反应与CBA小鼠相似，尽管在个体动物之间有所不同，并且与它们的背景fMPCE没有相关性。本研究表明，棕背䶄和田鼠在体内微核试验中是不合适的测试对象。另一方面，黄颈姬鼠和林姬鼠在该试验中似乎是有用的。由于野生姬鼠个体之间的差异相当大，当暴露于遗传毒性剂的剂量较低时，需要大群体才能获得具有统计学意义的结果。或者，可以从个体野生小鼠身上反复取样，以研究在将动物置于未受污染环境中一段时间后暴露减少的影响。
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