Heckmann S, Schliwa M, Kube-Granderath E
Adolf-Butenandt-Institute/Cell Biology, Munich, Germany.
Gene. 1997 Oct 15;199(1-2):303-9. doi: 10.1016/s0378-1119(97)00410-1.
We report the isolation and characterization of a cDNA-clone for gamma-tubulin from N. crassa. The deduced amino-acid sequence of the cDNA-clone most resembles that of gamma-tubulins from other fungi. Differential Southern blot analysis demonstrates that most probably a single gene represents gamma-tubulin. In addition, Northern blot analysis shows that most probably only one single mRNA encoding gamma-tubulin is transcribed in N. crassa. In a phylogenetic tree that includes all known gamma-tubulins, N. crassa gamma-tubulin is grouped, as expected, together with other fungal gamma-tubulins.
我们报道了来自粗糙脉孢菌的γ-微管蛋白cDNA克隆的分离和鉴定。该cDNA克隆推导的氨基酸序列与其他真菌的γ-微管蛋白最为相似。差异Southern印迹分析表明，很可能只有一个基因代表γ-微管蛋白。此外，Northern印迹分析表明，在粗糙脉孢菌中很可能只有一种编码γ-微管蛋白的单一mRNA被转录。在包含所有已知γ-微管蛋白的系统发育树中，粗糙脉孢菌的γ-微管蛋白正如预期的那样，与其他真菌的γ-微管蛋白归为一类。
