Müller T H, Döscher A, Schunter F
German Red Cross Blood Transfusion Service, Institute Oldenburg, Germany.
Vox Sang. 1997;73(3):185-8. doi: 10.1046/j.1423-0410.1997.7330185.x.
The purpose of the study was to establish a valid and efficient method for genotyping of the human platelet alloantigen-1 (HPA-1) in large sample numbers.
Digoxigenin- and fluorescein-labelled allele-specific primers and a biotinylated common primer were included in the hot-start PCR. Amplicons were bound to avidin-coated plates to identify the products by ELISA.
This approach reduced the number of PCR analyses for HPA-1 typing by half. PCR products were detected with high sensitivity and good reproducibility (interassay-CV: < 8%) in the ELISA. The typing of 100 blood donors with both PCR plus gel electrophoresis and ELISA-PCR showed identical results.
This method offers efficient HPA-1 genotyping in large numbers of donors and patients.
