Lakowicz J R, Gryczynski I, Nowaczyk K
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
Spectrochim Acta A Mol Biomol Spectrosc. 1997 Sep;53A(10):1637-44. doi: 10.1016/s1386-1425(97)00095-4.
We observed emission from the non-aromatic hydrocarbon 1,4-dioxane upon illumination with ps pulses at 380 nm. The emission intensity depended quadratically on incident power at 380 nm, indicating a two-photon process. In the absence of water the intensity decay was close to a single exponential, but displayed some evidence of an excited state process. In the presence of 1% water the emission spectra shifted dramatically to long wavelength. Water also resulted in wavelength-dependent intensity decays with negative pre-exponential factors on the long wavelength side of the emission, demonstrating the presence of an excited state reaction. At this water concentration the results are consistent with a two-state model due to emission from dioxane and a dioxane and a dioxane-water complex.
我们观察到，在380 nm处用皮秒脉冲照射时，非芳香烃1,4 - 二氧六环会发出荧光。发射强度与380 nm处的入射功率呈二次方关系，表明这是一个双光子过程。在无水情况下，强度衰减接近单指数衰减，但显示出一些激发态过程的迹象。在存在1%水的情况下，发射光谱显著向长波长方向移动。水还导致发射强度随波长衰减，在发射的长波长侧具有负的指数前因子，这表明存在激发态反应。在这种水浓度下，由于二氧六环以及二氧六环 - 水络合物的发射，结果与双态模型一致。
