Gryczynski I, Razynska A, Lakowicz J R
Department of Biological Chemistry, University of Maryland, School of Medicine, Baltimore 21201, USA.
Biophys Chem. 1996 Jan;57(2-3):291-5. doi: 10.1016/0301-4622(95)00066-4.
We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation. The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.
我们测量了直链烷烃十四烷在300nm激发下双光子激发的荧光发射光谱和强度衰减。纯溶液中十四烷的未猝灭寿命接近4.4纳秒。十四烷在210nm处的发射受到氧气、正丙醇和水的碰撞猝灭。这些结果表明，非极性环境中的脂肪族基团可以表现出良好的荧光，并且洗涤剂和脂质的脂肪族侧链可以作为胶束和双层膜的固有荧光探针。
