血浆和尿液中秋水仙碱的放射免疫分析

Radioimmunoassay for colchicine in plasma and urine.

作者信息

Ertel N H, Mittler J C, Akgun S, Wallace S L

出版信息

Science. 1976 Jul 16;193(4249):233-5. doi: 10.1126/science.935866.

Abstract

A radioimmunoassay for the measurement of colchicine (in quantities as small as 0.05 nanogram) in plasma and urine was developed with the use of an antibody from immunized rabbits. After the intravenous injection of 2 milligrams of colchicine in seven subjects, the calculated zero-time concentration in the plasma was 2.9 +/- 1.5 micrograms per deciliter, and the mean half-time in the plasma was 58 +/- 20 minutes. Declining, but measurable, amounts of colchicine could be detected in urine up to day 9 after the drug was administered.

摘要

利用免疫兔子产生的抗体，开发了一种用于测量血浆和尿液中秋水仙碱（含量低至0.05纳克）的放射免疫分析法。在7名受试者静脉注射2毫克秋水仙碱后，计算得出血浆中的零时间浓度为每分升2.9±1.5微克，血浆中的平均半衰期为58±20分钟。给药后直至第9天，尿液中均可检测到秋水仙碱含量呈下降趋势但仍可测量。