Draghia-Akli R, Li X, Schwartz R J
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Nat Biotechnol. 1997 Nov;15(12):1285-9. doi: 10.1038/nbt1197-1285.
Ectopic expression of a truncated growth hormone-releasing hormone (GHRH) from muscle tissues by a myogenic plasmid DNA vector directed by the skeletal alpha-actin promoter, pSK-GHRH, results in growth hormone (GH) secretion. Skeletal muscle-secreted GHRH is biologically active. The application of conditioned media harvested from pSK-GHRH transfected muscle cells to cultured pig primary anterior pituitary cells elicits GH release. A single intramuscular injection of 100 micrograms pSK-GHRH DNA elevates serum GH levels threefold to fourfold for up to 2 weeks, enhancing liver IGF-1 gene expression and increasing body weight approximately 10%.
由骨骼肌α-肌动蛋白启动子指导的肌源性质粒DNA载体pSK-GHRH在肌肉组织中异位表达截短的生长激素释放激素（GHRH），可导致生长激素（GH）分泌。骨骼肌分泌的GHRH具有生物活性。将从pSK-GHRH转染的肌肉细胞收获的条件培养基应用于培养的猪原代垂体前叶细胞，可引发GH释放。单次肌内注射100微克pSK-GHRH DNA可使血清GH水平在长达2周的时间内升高3至4倍，增强肝脏IGF-1基因表达，并使体重增加约10%。
