White N M, Bownes M
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland, UK.
Insect Mol Biol. 1997 Nov;6(4):329-41. doi: 10.1046/j.1365-2583.1997.00187.x.
The yolk protein (yp) genes encode the major nutritional polypeptides deposited in developing oocytes for subsequent utilization during embryogenesis, and represent a highly conserved family of genes in higher Diptera. Originally isolated from Drosophila melanogaster, they are expressed in a temporal-, tissue- and sex-specific manner in all species in which they have been identified. We report here the isolation of cDNAs encoding three independent yolk proteins from the common housefly, Musca domestica. Expression of the three M. domestica yp genes is analysed both by Northern and in-situ hybridization. We discuss in an evolutionary context both the significance of the expression patterns, and regions of apparent polypeptide sequence divergence.
卵黄蛋白（yp）基因编码在发育中的卵母细胞中沉积的主要营养多肽，以供胚胎发育期间后续利用，并且在高等双翅目中代表一个高度保守的基因家族。最初从黑腹果蝇中分离出来，它们在所鉴定的所有物种中以时间、组织和性别特异性的方式表达。我们在此报告从家蝇（Musca domestica）中分离出编码三种独立卵黄蛋白的cDNA。通过Northern杂交和原位杂交分析了三种家蝇yp基因的表达。我们在进化背景下讨论了表达模式的意义以及明显的多肽序列差异区域。
