Berger C, McQuain C, Sullivan J L, Nadal D, Quesenberry P J, Knecht H
Department of Pediatrics, University of Massachusetts Medical Center, Worcester 01655-0246, USA.
J Infect Dis. 1997 Nov;176(5):1370-3. doi: 10.1086/517324.
To assess the frequency of malignancy-associated 30-bp deletion variants of the latent membrane protein 1 (LMP-1) in benign conditions, a comparative sequence analysis was done using samples from 20 American children with acute infectious mononucleosis and 16 Swiss children with chronic tonsillar hyperplasia. The 30-bp deletion variant (LMP-1-del) was present in 66% of patients (12/20 with infectious mononucleosis and 12/16 with tonsillar hyperplasia). Two additional patients had a 3-bp deletion and an inframe insertion of 18 nucleotides, respectively. All but 1 isolate had numerous nonsilent point mutations. These data identify a hypervariable region within the C-terminus of LMP-1, in a domain required for maximal stimulation of NF-kappaB activity. These data demonstrate that LMP-1-del variants are frequent in acute infectious mononucleosis and tonsillar hyperplasia and identical to those observed in Epstein-Barr virus-associated AIDS-related lymphoma.
为评估潜伏膜蛋白1（LMP-1）的恶性肿瘤相关30碱基对缺失变异体在良性疾病中的出现频率，我们使用来自20名美国急性传染性单核细胞增多症儿童和16名瑞士慢性扁桃体增生儿童的样本进行了比较序列分析。30碱基对缺失变异体（LMP-1-del）存在于66%的患者中（传染性单核细胞增多症患者中12/20，扁桃体增生患者中12/16）。另外两名患者分别有一个3碱基对缺失和一个18个核苷酸的框内插入。除1个分离株外，所有分离株都有许多非同义点突变。这些数据确定了LMP-1 C末端内的一个高变区，该区域是最大程度刺激NF-κB活性所需的结构域。这些数据表明，LMP-1-del变异体在急性传染性单核细胞增多症和扁桃体增生中很常见，且与在爱泼斯坦-巴尔病毒相关的艾滋病相关淋巴瘤中观察到的变异体相同。
