Ertugrul N, Rodriguez-Barradas M C, Musher D M, Ryan M A, Agin C S, Murphy S J, Shayegani M, Watson D A
Veterans Affairs Medical Center, and Department of Medicine, Baylor College of Medicine, Houston, Texas 77030-4211, USA.
J Infect Dis. 1997 Nov;176(5):1401-5. doi: 10.1086/517331.
Nonserotypeable isolates predominate in epidemic conjunctivitis caused by Streptococcus pneumoniae. Previous evaluations of outbreaks of pneumococcal conjunctivitis have relied on epidemiologic factors and the nontypeability of the isolates to infer that a single clone was involved. In the present study, BOX-polymerase chain reaction DNA analysis was used to characterize nonserotypeable S. pneumoniae isolated by conjunctival culture during a recent conjunctivitis outbreak and to compare these isolates with those from outbreaks described earlier. The recent outbreak was caused by a single pneumococcal clone. Outbreaks in separate parts of the United States in 1980-1981 were all caused by the same clone. Cluster analysis revealed a high degree of genetic relatedness among isolates causing conjunctivitis compared with that among other nonserotypeable S. pneumoniae, with the closest relatedness being found among the 1996 and 1980-1981 conjunctival isolates.
在由肺炎链球菌引起的流行性结膜炎中，不可分型菌株占主导地位。以往对肺炎球菌性结膜炎暴发的评估依赖于流行病学因素以及菌株的不可分型性，以此推断涉及单一克隆。在本研究中，采用BOX聚合酶链反应DNA分析对近期结膜炎暴发期间通过结膜培养分离出的不可分型肺炎链球菌进行特征分析，并将这些分离株与早期描述的暴发中的分离株进行比较。近期的暴发是由单一肺炎球菌克隆引起的。1980 - 1981年在美国不同地区的暴发均由同一克隆引起。聚类分析显示，与其他不可分型肺炎链球菌相比，引起结膜炎的分离株之间存在高度的遗传相关性，其中1996年和1980 - 1981年的结膜分离株之间的相关性最为密切。
