Akiba T, Ota T, Fushimi K, Tamura H, Hata T, Sasaki S, Marumo F
Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Adv Perit Dial. 1997;13:3-6.
To clarify the mechanism of water transport driven by osmotic gradient through "ultrasmall pores" in the peritoneum, we tried to identify water channels in the peritoneum and cells in the peritoneal dialysate. Peritoneum was surgically excised from uremic patients at the insertion or removal of a catheter. Sediment was collected from 2 L of peritoneal dialysate by centrifugation at 1500 rpm. RNA was extracted and amplified by reverse transcription-polymerase chain reaction (RT-PCR). Contamination of reticulocytes was tested by the presence of ankyrin mRNA. Peritoneal tissue expressed aquaporin (AQP) 1, 3, and 4 (AQP1 > 3 > 4). Sediment of dialysate expressed mRNA of AQP1 and AQP3 (AQP1 > AQP3). The sample did not express ankyrin mRNA, indicating that the AQP1 in the sediment did not originate from reticulocytes. These data indicate that aquaporins are present in the peritoneum and might participate in water transport. Further quantitative analysis of aquaporin messages in the dialysate might clarify the pathogenesis of water removal failure.
为了阐明渗透梯度驱动水通过腹膜“超小孔”进行转运的机制，我们试图鉴定腹膜中的水通道以及腹膜透析液中的细胞。在插入或拔除导管时，从尿毒症患者身上手术切除腹膜。通过以1500转/分钟的转速离心2L腹膜透析液来收集沉淀物。提取RNA并通过逆转录-聚合酶链反应（RT-PCR）进行扩增。通过锚蛋白mRNA的存在来检测网织红细胞的污染情况。腹膜组织表达水通道蛋白（AQP）1、3和4（AQP1 > 3 > 4）。透析液沉淀物表达AQP1和AQP3的mRNA（AQP1 > AQP3）。样本未表达锚蛋白mRNA，这表明沉淀物中的AQP1并非源自网织红细胞。这些数据表明水通道蛋白存在于腹膜中，并且可能参与水的转运。对透析液中水通道蛋白信息进行进一步的定量分析可能会阐明水清除失败的发病机制。
