编码线粒体核糖体蛋白的酵母NAM9基因的表达。
Expression of the yeast NAM9 gene coding for mitochondrial ribosomal protein.
作者信息
Boguta M, Chacińska A, Murawski M, Szcześniak B
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
出版信息
Acta Biochim Pol. 1997;44(2):251-8.
We studied expression of the NAM9 gene of Saccharomyces cerevisiae that was previously reported to code for a mitochondrial ribosomal protein. Increase in NAM9 gene dosage is accompanied by the increase in both mRNA and protein. The levels of the NAM9 transcript and protein are both reduced in cells growing on glucose as compared to cells growing on galactose as a carbon source. Nam9p accumulates to the same level in rho(o) and rho(+) cells. These results confirm previous data indicating diverse regulation of different mitochondrial ribosomal protein genes and suggest that expression of Nam9p is not co-ordinated with the expression of other mitochondrial ribosomal components.
我们研究了酿酒酵母的NAM9基因的表达，该基因先前报道编码一种线粒体核糖体蛋白。NAM9基因剂量的增加伴随着mRNA和蛋白质的增加。与以半乳糖作为碳源生长的细胞相比，在葡萄糖上生长的细胞中NAM9转录本和蛋白质的水平均降低。Nam9p在ρ（0）和ρ（+）细胞中积累到相同水平。这些结果证实了先前的数据，表明不同的线粒体核糖体蛋白基因受到不同的调控，并表明Nam9p的表达与其他线粒体核糖体成分的表达不协调。
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