Vaden S L, Gookin J, Trogdon M, Langston C E, Levine J, Cowgill L D
Department of Companion Animal and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Am J Vet Res. 1997 Nov;58(11):1193-6.
To determine usefulness of carbamylated hemoglobin (CarHb) concentration for differentiation of acute renal failure (ARF) from chronic renal failure (CRF) in dogs.
Samples from dogs with ARF or CRF and from nonazotemic control dogs.
CarHb concentration was determined in heparinized blood samples by measuring the micrograms of valine hydantoin (VH) per gram of hemoglobin (Hb), using a high-performance liquid chromatography assay, in which carbamyl valine is converted to VH via acid hydrolysis.
CarHb concentration was significantly higher in dogs with ARF and CRF, compared with values in control dogs (ARF vs control, P < 0.05; CRF vs control, P < 0.001). Furthermore, CarHb concentration was significantly (P < 0.001) higher in dogs with CRF, compared with that in dogs with ARF. Carbamylated hemoglobin concentration did not correlate with serum urea nitrogen or creatinine concentration. Using a cutoff value of 100 micrograms of VH/g of Hb, the sensitivity and specificity of CarHb concentration for differentiating ARF from CRF was 96.1 and 84.2%, respectively.
CarHb concentration was useful in the differentiation of ARF from CRF in the dogs of this study.
CarHb concentration may be used to increase the accuracy of identifying ARF, so that early, aggressive management can be instituted, thereby increasing the chance of recovery.
确定氨甲酰化血红蛋白（CarHb）浓度对鉴别犬急性肾衰竭（ARF）和慢性肾衰竭（CRF）的作用。
来自患有ARF或CRF的犬以及非氮血症对照犬的样本。
通过使用高效液相色谱分析法，测量每克血红蛋白（Hb）中缬氨酸乙内酰脲（VH）的微克数，来测定肝素化血样中的CarHb浓度，其中氨甲酰缬氨酸通过酸水解转化为VH。
与对照犬的值相比，患有ARF和CRF的犬的CarHb浓度显著更高（ARF与对照相比，P < 0.05；CRF与对照相比，P < 0.001）。此外，与患有ARF的犬相比，患有CRF的犬的CarHb浓度显著更高（P < 0.001）。氨甲酰化血红蛋白浓度与血清尿素氮或肌酐浓度无关。使用100微克VH/g Hb的临界值，CarHb浓度区分ARF和CRF的敏感性和特异性分别为96.1%和84.2%。
在本研究的犬中，CarHb浓度有助于鉴别ARF和CRF。
CarHb浓度可用于提高识别ARF的准确性，以便能够进行早期积极治疗，从而增加恢复的机会。
