Kwan J T, Carr E C, Bending M R, Barron J L
South West Thames Regional Renal Unit, St. Helier Hospital, Carshalton, Surrey, U.K.
Clin Chem. 1990 Apr;36(4):607-10.
We have developed an HPLC method for measuring carbamylated hemoglobin (CarHb), based on the quantification of valine hydantoin formed from the released NH2-terminal carbamyl valine residue after acid hydrolysis of hemoglobin. In uremia, CarHb is produced by nonenzymatic post-translational modification of the terminal amino group of hemoglobin monomers by isocyanic acid, derived from the spontaneous dissociation of urea. We measured CarHb in 25 nonuremic control subjects, 24 nonuremic diabetic subjects, and 30 patients with stable chronic renal failure. There was no significant difference between the controls and diabetic patients, their mean (SD) CarHb values being 41 (11.5) and 38 (10.8) micrograms of carbamyl valine per gram of hemoglobin (microgram CV/gHb), respectively. Mean (SD) CarHb values in the uremic patients were much greater, 164 (87.7) microgram CV/gHb. There was significant correlation between the concentrations of CarHb and plasma urea in the uremic subjects. Thus CarHb provides a urea-derived index of chronic uremia.
我们开发了一种用于测量氨甲酰化血红蛋白（CarHb）的高效液相色谱法，该方法基于对血红蛋白酸水解后释放的NH2-末端氨甲酰缬氨酸残基形成的缬氨酸乙内酰脲进行定量。在尿毒症中，CarHb是由血红蛋白单体的末端氨基通过异氰酸进行非酶促翻译后修饰产生的，异氰酸源自尿素的自发解离。我们测量了25名非尿毒症对照受试者、24名非尿毒症糖尿病受试者和30名稳定的慢性肾衰竭患者的CarHb。对照组和糖尿病患者之间没有显著差异，他们的平均（标准差）CarHb值分别为每克血红蛋白41（11.5）和38（10.8）微克氨甲酰缬氨酸（微克CV/gHb）。尿毒症患者的平均（标准差）CarHb值要高得多，为164（87.7）微克CV/gHb。在尿毒症受试者中，CarHb浓度与血浆尿素之间存在显著相关性。因此，CarHb提供了一种源自尿素的慢性尿毒症指标。
