Shen L, van Dyck K, Luten J, Deelstra H
Department of Pharmaceutical Sciences, University of Antwerp (UIA), Belgium.
Biol Trace Elem Res. 1997 Jul-Aug;58(1-2):55-63. doi: 10.1007/BF02910666.
The present study was undertaken to evaluate the in vitro availability of chemically varying forms of selenium (Se), supplemented in cow's milk. Two inorganic (selenite and selenate) and two organic (seleno-methionine [Se-Met] and seleno-cystine [Se-Cys]) Se sources were evaluated. The in vitro availability was estimated by the diffusibility of Se during simulated gastrointestinal digestion. First, the diffusibility was compared after adding a constant amount of Se as either selenate, selenite, seleno-methionine, or Se-Cys in milk samples. Se-Met and selenate were found to be significantly more diffusible than seleno-cystine and selenite under the simulated gastrointestinal conditions. The tendency for superior in vitro availability of selenate and Se-Met compared to selenite and Se-Cys was confirmed for a supplementation range of 5-40 ng/g of Se. This study suggests that the high diffusibility of selenate and Se-Met in a simulated gastrointestinal environment may contribute to their high absorption in vivo.
本研究旨在评估添加到牛奶中的化学形态各异的硒（Se）的体外可利用性。评估了两种无机硒源（亚硒酸盐和硒酸盐）和两种有机硒源（硒代蛋氨酸[Se-Met]和硒代胱氨酸[Se-Cys]）。通过模拟胃肠道消化过程中硒的扩散性来估计体外可利用性。首先，在牛奶样品中添加等量的硒，分别为硒酸盐、亚硒酸盐、硒代蛋氨酸或硒代胱氨酸，然后比较其扩散性。发现在模拟胃肠道条件下，硒代蛋氨酸和硒酸盐的扩散性明显高于硒代胱氨酸和亚硒酸盐。对于5-40 ng/g的硒添加范围，证实了硒酸盐和硒代蛋氨酸相比亚硒酸盐和硒代胱氨酸具有更高的体外可利用性。本研究表明，硒酸盐和硒代蛋氨酸在模拟胃肠道环境中的高扩散性可能有助于它们在体内的高吸收。
