Zoroddu M A, Masia A
Dipartimento di Chimica, Università di Sassari, Italy.
Biochim Biophys Acta. 1997 Oct 11;1358(3):249-54. doi: 10.1016/s0167-4889(97)00074-8.
Saccharomyces cerevisiae cells stored oxovanadium (IV) ions in a dimeric form. In the late stationary phase Saccharomyces cerevisiae cells grown in rich medium containing concentrations of oxovanadium (V), orthovanadate from 12 to 18 mM, causing growth stasis, a dimeric oxovanadium (IV) species was identified by EPR spectroscopy. The EPR spectrum exhibited at 110 K the low-field forbidden deltaMs = +/-2 transition at g around 4 and the half-field deltaMs = +/-1 15-lines feature at g around 2 out of the presence of a triplet state by the coupling of the oxovanadium (IV) ions in a dimeric form. Hyperfine splitting of 75.2 x 10(-4) cm(-1) and an interionic distance of about 4.4 angstroms was calculated. The dimeric species was localized in the cellular cytoplasmic space.
酿酒酵母细胞以二聚体形式储存氧钒（IV）离子。在含有12至18 mM浓度的氧钒（V）、原钒酸盐的丰富培养基中生长的酿酒酵母细胞，在稳定期末期会导致生长停滞，通过电子顺磁共振光谱法鉴定出一种二聚体氧钒（IV）物种。电子顺磁共振光谱在110 K时显示，由于二聚体形式的氧钒（IV）离子的耦合存在三重态，在g约为4处有低场禁戒的δMs = +/-2跃迁，在g约为2处有半场δMs = +/-1的15线特征。计算出超精细分裂为75.2 x 10(-4) cm(-1)，离子间距离约为4.4埃。二聚体物种定位于细胞胞质空间。
