Garraud O, Perler F B, Bradley J E, Nutman T B
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20850, USA.
J Immunol. 1997 Nov 15;159(10):4793-8.
Using two recombinant filarial protein Ags and keyhole limpet hemocyanin, we sensitized T cells from uninfected, nonatopic individuals in such a manner that they were able to provide help for the selective induction of an Ag-specific Ab response. IL-2 and IL-4 were shown to be critical for sensitizing the T cells; once sensitized, these T cells could provide the necessary signals for B cells to produce Ag-specific Abs, provided that IL-4 (or IL-2) was supplied exogenously. Primary exposure of T cells to IFN-gamma, but not to IL-12, prevented the Ag-sensitized T cells from helping B cells to produce specific Abs, apart from the IgG2 isotype. These data suggest that Ab-producing B cells of a defined Ag specificity and isotype can be generated differentially after in vitro priming of human T cells by Ag, providing regulatory cytokines are also present.
我们使用两种重组丝虫蛋白抗原和匙孔血蓝蛋白，以这样一种方式使未感染、非特应性个体的T细胞致敏，即它们能够为选择性诱导抗原特异性抗体反应提供帮助。白细胞介素-2（IL-2）和白细胞介素-4被证明对T细胞致敏至关重要；一旦致敏，这些T细胞可以为B细胞产生抗原特异性抗体提供必要信号，前提是外源性提供白细胞介素-4（或白细胞介素-2）。T细胞初次暴露于干扰素-γ（IFN-γ）而非白细胞介素-12，除IgG2亚型外，会阻止抗原致敏的T细胞帮助B细胞产生特异性抗体。这些数据表明，在体外通过抗原致敏人类T细胞后，如果也存在调节性细胞因子，具有特定抗原特异性和亚型的产生抗体的B细胞可以以不同方式产生。
