Olsson N U, Kaufmann P, Dzeletović S
Scotia LipidTeknik AB, Stockholm, Sweden.
J Chromatogr B Biomed Sci Appl. 1997 Sep 26;698(1-2):1-8. doi: 10.1016/s0378-4347(97)00300-9.
A procedure for the preparation of long-chain base residues of egg yolk, bovine milk and bovine brain sphingomyelin was developed. The bases were converted to N-acetyl-O-trimethylsilyl (TMS) derivatives before being submitted to gas chromatography and mass spectrometry. The chromatographic profile of the milk sample was complex with thirteen peaks, whereas the profiles of brain and egg yolk long-chain bases were simple and remarkably similar.
开发了一种制备蛋黄、牛乳和牛脑鞘磷脂长链碱基残基的方法。在进行气相色谱和质谱分析之前，将这些碱基转化为N-乙酰基-O-三甲基硅烷基（TMS）衍生物。牛奶样品的色谱图很复杂，有13个峰，而脑和蛋黄长链碱基的色谱图则很简单且非常相似。
