Smits P, Ni J, Feng P, Wauters J, Van Hul W, Boutaibi M E, Dillon P J, Merregaert J
Department of Biochemistry, Department of Medicine, Universitaire Instelling Antwerpen, Universiteitsplein 1, Wilrijk, 2610, Belgium.
Genomics. 1997 Nov 1;45(3):487-95. doi: 10.1006/geno.1997.4918.
The Ecm1 gene encodes an 85-kDa protein of unknown function that was originally identified as a secretory protein of the murine osteogenic stromal cell line MN7. This paper describes the isolation of a genomic clone containing the human ECM1 gene from a chromosome 1 cosmid library and the characterization of its exon-intron structure. The protein coding region comprises 10 exons. The ECM1 gene maps at chromosome 1q21 outside the epidermal differentiation complex region. The ECM1 gene appears to be expressed as a 1.8-kb transcript predominantly in placenta and heart, while an additional 1.4-kb alternatively spliced message was detected in tonsils. The full-length human ECM1 transcript contains 1838 bp and has an overall homology of 79.6% with the mouse Ecm1 cDNA. This transcript codes for a protein of 540 amino acids that is 69.4% identical and 81.3% similar to the corresponding mouse protein. The alternatively spliced variant, 1450 bp long, encodes a protein of 415 amino acids.
Ecm1基因编码一种功能未知的85 kDa蛋白质，该蛋白质最初被鉴定为小鼠成骨基质细胞系MN7的分泌蛋白。本文描述了从1号染色体黏粒文库中分离出包含人ECM1基因的基因组克隆及其外显子-内含子结构的特征。蛋白质编码区由10个外显子组成。ECM1基因定位于1号染色体q21上，在表皮分化复合体区域之外。ECM1基因似乎以1.8 kb的转录本形式主要在胎盘和心脏中表达，而在扁桃体中检测到另外一种1.4 kb的可变剪接转录本。全长人ECM1转录本包含1838 bp，与小鼠Ecm1 cDNA的总体同源性为79.6%。该转录本编码一种540个氨基酸的蛋白质，与相应的小鼠蛋白质的同一性为69.4%，相似性为81.3%。可变剪接变体长度为1450 bp，编码一种415个氨基酸的蛋白质。
