Jacoby A S, Webb G C, Liu M L, Kofler B, Hort Y J, Fathi Z, Bottema C D, Shine J, Iismaa T P
Neurobiology Program, The Garvan Institute of Medical Research, 384 Victoria Street, Sydney, New South Wales, 2010, Australia.
Genomics. 1997 Nov 1;45(3):496-508. doi: 10.1006/geno.1997.4960.
The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Human and rat GALR1 galanin receptor cDNA clones have previously been isolated using expression cloning. We have used the human GALR1 cDNA in hybridization screening to isolate the gene encoding GALR1 in both human (GALNR) and mouse (Galnr). The gene spans approximately 15-20 kb in both species; its structural organization is conserved and is unique among G-protein-coupled receptors. The coding sequence is contained on three exons, with exon 1 encoding the N-terminal end of the receptor and the first five transmembrane domains. Exon 2 encodes the third intracellular loop, while exon 3 encodes the remainder of the receptor, from transmembrane domain 6 to the C-terminus of the receptor protein. The mouse and human GALR1 receptor proteins are 348 and 349 amino acids long, respectively, and display 93% identity at the amino acid level. The mouse Galnr gene has been localized to Chromosome 18E4, homoeologous with the previously reported localization of the human GALNR gene to 18q23 in the same syntenic group as the genes encoding nuclear factor of activated T-cells, cytoplasmic 1, and myelin basic protein.
神经肽甘丙肽通过与特定的G蛋白偶联受体相互作用引发一系列生物学效应。此前已利用表达克隆技术分离出人类和大鼠的GALR1甘丙肽受体cDNA克隆。我们使用人类GALR1 cDNA进行杂交筛选，以分离人类（GALNR）和小鼠（Galnr）中编码GALR1的基因。在这两个物种中，该基因跨度约为15 - 20 kb；其结构组织保守，在G蛋白偶联受体中独一无二。编码序列包含在三个外显子上，外显子1编码受体的N末端和前五个跨膜结构域。外显子2编码第三个细胞内环，而外显子3编码受体的其余部分，从跨膜结构域6到受体蛋白的C末端。小鼠和人类GALR1受体蛋白分别由348和349个氨基酸组成，在氨基酸水平上具有93%的同一性。小鼠Galnr基因已定位到18号染色体E4区，与先前报道的人类GALNR基因定位到18q23同属一个同线群，该同线群还包括编码活化T细胞核因子、细胞质1和髓鞘碱性蛋白的基因。
