Carmeci C, Thompson D A, Ring H Z, Francke U, Weigel R J
Department of Surgery, Stanford University, Stanford, California, 94305, USA.
Genomics. 1997 Nov 1;45(3):607-17. doi: 10.1006/geno.1997.4972.
Using the technique of differential cDNA library screening, a cDNA clone was isolated from an estrogen receptor (ER)-positive breast carcinoma cell line (MCF7) cDNA library based upon the overexpression of this gene compared to an ER-negative cell line (MDA-MB-231). Sequence analysis of this clone determined that it shared significant homology to G-protein-coupled receptors. This receptor, GPCR-Br, was abundantly expressed in the ER-positive breast carcinoma cell lines MCF7, T-47D, and MDA-MB-361. Expression was absent or minimal in the ER-negative breast carcinoma cell lines BT-20, MDA-MB-231, and HBL-100. GPCR-Br was ubiquitously expressed in human tissues examined but was most abundant in placenta. GPCR-Br expression was examined in 11 primary breast carcinomas. GPCR-Br was detected in all 4 ER-positive tumors and only 1 of 7 ER-negative tumors. Based upon PCR analysis in hybrid cell lines, the gene for GPCR-Br (HGMW-approved symbol GPR30) was mapped to chromosome 7p22. The pattern of expression of GPCR-Br indicates that this receptor may be involved in physiologic responses specific to hormonally responsive tissues.
利用差异cDNA文库筛选技术，基于与雌激素受体（ER）阴性细胞系（MDA-MB-231）相比该基因的过表达，从雌激素受体（ER）阳性乳腺癌细胞系（MCF7）cDNA文库中分离出一个cDNA克隆。对该克隆的序列分析确定，它与G蛋白偶联受体具有显著同源性。这种受体，即GPCR-Br，在ER阳性乳腺癌细胞系MCF7、T-47D和MDA-MB-361中大量表达。在ER阴性乳腺癌细胞系BT-20、MDA-MB-231和HBL-100中表达缺失或极少。GPCR-Br在所检测的人体组织中普遍表达，但在胎盘中最为丰富。对11例原发性乳腺癌进行了GPCR-Br表达检测。在所有4例ER阳性肿瘤中均检测到GPCR-Br，而在7例ER阴性肿瘤中仅1例检测到。基于对杂交细胞系的PCR分析，GPCR-Br基因（HGMW认可符号为GPR30）被定位于7号染色体p22区域。GPCR-Br的表达模式表明，该受体可能参与了激素反应性组织特有的生理反应。
